Lauric Acid (Dodecylic acid),which is found naturally in various plant and animal fats and oils, is a saturated medium-chain fatty acid with a 12-carbon backbone. It is a major component of coconut oil and palm kernel oil.

S. epidermidis:4 μg/mL(EC50), P. acnes:2μg/mL(EC50), S. aureus:6μg/mL(EC50)

Lauric acid在Caco-2细胞（p<0.05）和IEC-6细胞（p<0.05）中诱导凋亡。在Caco-2细胞中，Lauric acid降低了GSH的可用性并且与丁酸比较，产生了更多的ROS（p < 0.05）。Lauric acid使得Caco-2和IEC-6细胞在G0/G1期的数量减少，同时在S期和G2/M期阶段停滞。与丁酸相比，Lauric acid在IEC-6细胞中诱导了凋亡（p < 0.05），并引起了高水平的ROS[1]。

JAK2 and other human kinase activity assays: JAK2 kinase activity was assessed with the HTScan JAK2 Kinase Assay Kit per manufacturer's protocol. The possible effects of FLLL32 on the other 10 purified human protein kinases were determined by using a Kinase Profiler Assay.

Cell lines are harvested by enzymatic dissociation and seeded into 24-well tissue culture plates at 2.5 × 105 and 1.5 × 105 cells/ml, respectively. After 24 h of incubation, the medium is replaced with 1 ml of complete DMEM containing freshly prepared (50 mM )Lauric acid, conjugated to 0.4% FAF-BSA to final concentrations of 0.1, 0.3, 0.5 and 1 mM and compared to NaB (5 mM ) with 50 μl of 0.4% FAF-BSA as a vehicle control and incubated in a humidified atmosphere at 37℃ in 5% CO2 and, after enzymatic dissociation, assayed at 24, 48, 72, and 96 h. All experimental studies are undertaken in triplicate and measured in duplicate. (Only for Reference)